Coding
Part:BBa_K2114005:Design
Designed by: Wladislaw Stroukov Group: iGEM16_Freiburg (2016-10-08)
GST_HA_G4S_CotZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 792
Illegal BamHI site found at 688 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 85
Design Notes
Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.
Source
The cotZ gene comes from the genome of B. subtilis. The glutathione S-transferase was amplified from the pGEX-6P-1 vector (GE healthcare).